geo affymetrix microarray pre processing steps Search Results


affymetrix arabidopsis dna chips  (Thermo Fisher)
99
Thermo Fisher affymetrix arabidopsis dna chips
FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from <t>Arabidopsis</t> roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.
Affymetrix Arabidopsis Dna Chips, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glyceraldehyde 3 phosphate dehydrogenase gapdh  (Thermo Fisher)
99
Thermo Fisher glyceraldehyde 3 phosphate dehydrogenase gapdh
FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from <t>Arabidopsis</t> roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.
Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray chips  (AECOM International Development)
90
AECOM International Development microarray chips
FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from <t>Arabidopsis</t> roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.
Microarray Chips, supplied by AECOM International Development, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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44k rice oligo microarray platform  (Thermo Fisher)
99
Thermo Fisher 44k rice oligo microarray platform
FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from <t>Arabidopsis</t> roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.
44k Rice Oligo Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cyp7b1 mm00484157 m1  (Thermo Fisher)
86
Thermo Fisher gene exp cyp7b1 mm00484157 m1
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Gene Exp Cyp7b1 Mm00484157 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aldosterone treatment  (Thermo Fisher)
95
Thermo Fisher aldosterone treatment
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Aldosterone Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hdl cholesterol  (Thermo Fisher)
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Thermo Fisher hdl cholesterol
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Hdl Cholesterol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna microarray  (Thermo Fisher)
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Thermo Fisher dna microarray
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Dna Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tax oryza sativa repid o82807 orysj 2 050 ta 19609 1 s1 at aj615628 cytochrome p450 n  (Thermo Fisher)
98
Thermo Fisher tax oryza sativa repid o82807 orysj 2 050 ta 19609 1 s1 at aj615628 cytochrome p450 n
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Tax Oryza Sativa Repid O82807 Orysj 2 050 Ta 19609 1 S1 At Aj615628 Cytochrome P450 N, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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affymetrix 428 scanner  (MWG-Biotech ag)
90
MWG-Biotech ag affymetrix 428 scanner
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Affymetrix 428 Scanner, supplied by MWG-Biotech ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray platforms  (Thermo Fisher)
99
Thermo Fisher microarray platforms
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Microarray Platforms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microarray platforms - by Bioz Stars, 2026-09
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genechip mouse genome 430 2.0 array  (LC Sciences)
90
LC Sciences genechip mouse genome 430 2.0 array
Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) <t>CYP7b1</t> mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.
Genechip Mouse Genome 430 2.0 Array, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from Arabidopsis roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.

Journal: Journal of Biological Chemistry

Article Title: Expression Profiles of Arabidopsis thaliana in Mineral Deficiencies Reveal Novel Transporters Involved in Metal Homeostasis

doi: 10.1074/jbc.m309338200

Figure Lengend Snippet: FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from Arabidopsis roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.

Article Snippet: Genome-wide Analysis Provides Insight into Metal Transport—We have used Affymetrix Arabidopsis DNA chips containing 8,300 genes (which cover about one-third of the ge- FIG. 7.

Techniques: Functional Assay, Hybridization, Control, Staining, Expressing, Mutagenesis, Transformation Assay, Plasmid Preparation

Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) CYP7b1 mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.

Journal: Cancer Science

Article Title: Carcinogenicity of dimethylarsinic acid in Ogg1 ‐deficient mice

doi: 10.1111/j.1349-7006.2007.00475.x

Figure Lengend Snippet: Gene Spring clustering analysis of the Affymetrix oligonucleotide microarray data (a) Ingenuity pathway analysis (b) and conformation of microarray results by real‐time quantitative polymerase chain reaction (Q‐PCR) (c–f). (c) Polymerase (DNA‐directed) alpha 1 (d) Mmp13 (e) nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha, and (f) CYP7b1 mRNA expression. *P < 0.05 and **P < 0.0001 versus Ogg1 +/+ control and dimethylarsinic acid (DMAV)‐treated groups.

Article Snippet: For the confirmation of Affimetrix microarray analysis results, real‐time quantitative‐polymerase chain reaction (Q‐PCR) was carried out using TaqMan probes for 490 C. Primer sequences were designed with Primer Express software (Applied Biosystems, USA): Mm00447106_m1 for polymerase (DNA‐directed), alpha 1 ( NM_008892 ); Mm00484157_m1 for cytochrome P450, 7b1 ( NM_007825 ); Mm00772875_m1 for nicotineamide adenine dinucleotide reduced form (NADH) dehydrogenase (ubiquinone) 1 alpha subcomplex, 3 ( NM_025348 ); Mm00439491_m1 for matrix metalloproteinase 13 ( NM_008607 ); Mm00834384_g1 for heat shock protein 1 ( NM_013560 ); and Mm00607939 for beta‐actin, cytoplasmic ( NM_007393 ).

Techniques: Microarray, Real-time Polymerase Chain Reaction, Expressing, Control